Green light for gene targeting in plants

Green light for gene targeting in plants.

P recision engineering of eukaryotic organisms requires efficient techniques for homology-based replacement of an endogenous gene by an introduced gene, a process termed gene targeting (GT). Because foreign DNA is preferentially integrated by nonhomologous recombination in plants and animals, special strategies are needed to increase the frequency and facilitate the detection of GT. Developing ...

Light Production by Green Plants

1. Green plants have been found to emit light of approximately the same color as their fluorescent light for several minutes following illumination. This light is about 10(-3) the intensity of the fluorescent light, about one-tenth second after illumination below saturation or 10(-6) of the intensity of the absorbed light. 2. The decay curve follows bimolecular kinetics at 6.5 degrees C. and re...

Gene targeting in plants: 25 years later.

Only five years after the initiation of transgenic research in plants, gene targeting (GT) was achieved for the first time in tobacco. Unfortunately, the frequency of targeted integration via homologous recombination (HR) was so low in comparison to random integration that GT could not be established as a feasible technique in higher plants. It took another 25 years and great effort to develop ...

Gene Targeting and Genetic Transformation of Plants

Discovery of second gene for solid dark green versus light green rind pattern in watermelon.

The watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus) has high variability for fruit size, shape, rind pattern, and flesh color. This study was designed to measure the qualitative inheritance of rind phenotypes (solid dark green vs. light green). For each of the 2 families, "Mountain Hoosier" × "Minilee" and "Early Arizona" × "Minilee," 6 generations (P(a)S(1), P(b)S(1), F(1)...